{
  "id": "732f7f7d-1575-5a5b-b45a-f8e408ba67f7",
  "slug": "d1-1-dna-replication",
  "title": "D1.1 DNA Replication",
  "course": "IB Biology",
  "level": "SL and HL",
  "syllabusCode": "D1.1",
  "questionCount": 18,
  "page": "https://splycr.com/ib-biology/d1-1-dna-replication",
  "data": "https://splycr.com/library/d1-1-dna-replication.json",
  "play": "https://splycr.com/?set=732f7f7d-1575-5a5b-b45a-f8e408ba67f7",
  "section": {
    "name": "Theme D: Continuity and change",
    "page": "https://splycr.com/ib-biology/continuity-and-change"
  },
  "source": {
    "name": "Biology by Bradford",
    "href": "https://biologybybradford.com"
  },
  "questions": [
    {
      "number": 1,
      "question": "The direction in which DNA Polymerase always adds new nucleotides?",
      "answer": "5' to 3' direction",
      "wrongOptions": [
        "Gel electrophoresis",
        "DNA Polymerase I",
        "Polymerase chain reaction (PCR)"
      ]
    },
    {
      "number": 2,
      "question": "The enzyme that relieves torsional strain (overwinding) ahead of the replication fork?",
      "answer": "DNA Gyrase (Topoisomerase)",
      "wrongOptions": [
        "Complementary base pairing",
        "Meselson-Stahl Experiment",
        "DNA Polymerase I"
      ]
    },
    {
      "number": 3,
      "question": "Proteins that bind to the DNA strands after they are separated to prevent them from re-annealing?",
      "answer": "Single-Strand Binding Proteins (SSBs)",
      "wrongOptions": [
        "Polymerase chain reaction (PCR)",
        "Complementary base pairing",
        "DNA Gyrase (Topoisomerase)"
      ]
    },
    {
      "number": 4,
      "question": "The enzyme that creates a short RNA primer to provide an attachment point for DNA Polymerase?",
      "answer": "DNA Primase",
      "wrongOptions": [
        "DNA Ligase",
        "Okazaki Fragments",
        "Leading Strand"
      ]
    },
    {
      "number": 5,
      "question": "The strand that is synthesized continuously moving toward the replication fork?",
      "answer": "Leading Strand",
      "wrongOptions": [
        "Okazaki Fragments",
        "Taq polymerase",
        "DNA Polymerase III"
      ]
    },
    {
      "number": 6,
      "question": "The strand that is synthesized discontinuously in fragments moving away from the fork?",
      "answer": "Lagging Strand",
      "wrongOptions": [
        "Taq polymerase",
        "Okazaki Fragments",
        "Leading Strand"
      ]
    },
    {
      "number": 7,
      "question": "Short lengths of DNA produced on the lagging strand?",
      "answer": "Okazaki Fragments",
      "wrongOptions": [
        "DNA Polymerase III",
        "Lagging Strand",
        "Leading Strand"
      ]
    },
    {
      "number": 8,
      "question": "The enzyme that joins Okazaki fragments together by forming phosphodiester bonds?",
      "answer": "DNA Ligase",
      "wrongOptions": [
        "DNA Primase",
        "Okazaki Fragments",
        "Taq polymerase"
      ]
    },
    {
      "number": 9,
      "question": "The enzyme that removes the RNA primers and replaces them with DNA?",
      "answer": "DNA Polymerase I",
      "wrongOptions": [
        "Leading Strand",
        "DNA Polymerase III",
        "Okazaki Fragments"
      ]
    },
    {
      "number": 10,
      "question": "The term describing DNA replication because each new molecule contains one original and one new strand?",
      "answer": "Semi-conservative",
      "wrongOptions": [
        "Centrifugation",
        "Lagging Strand",
        "DNA Polymerase III"
      ]
    },
    {
      "number": 11,
      "question": "The enzyme that unwinds the double helix and breaks hydrogen bonds between bases?",
      "answer": "Helicase",
      "wrongOptions": [
        "DNA Primase",
        "Centrifugation",
        "Semi-conservative"
      ]
    },
    {
      "number": 12,
      "question": "Main enzyme in prokaryotes that adds nucleotides to the 3' end of the primer to build the new strand?",
      "answer": "DNA Polymerase III",
      "wrongOptions": [
        "Okazaki Fragments",
        "Leading Strand",
        "Lagging Strand"
      ]
    },
    {
      "number": 13,
      "question": "The experiment that used ¹⁵N and ¹⁴N to show that DNA replication is semi-conservative?",
      "answer": "Meselson-Stahl Experiment",
      "wrongOptions": [
        "Gel electrophoresis",
        "DNA Gyrase (Topoisomerase)",
        "Complementary base pairing"
      ]
    },
    {
      "number": 14,
      "question": "The technique used in the Meselson-Stahl lab to separate DNA based on density?",
      "answer": "Centrifugation",
      "wrongOptions": [
        "Semi-conservative",
        "Helicase",
        "Lagging Strand"
      ]
    },
    {
      "number": 15,
      "question": "The way in which DNA bases pair: A with T and G with C?",
      "answer": "Complementary base pairing",
      "wrongOptions": [
        "DNA Gyrase (Topoisomerase)",
        "Meselson-Stahl Experiment",
        "Polymerase chain reaction (PCR)"
      ]
    },
    {
      "number": 16,
      "question": "What is the technique used to amplify DNA?",
      "answer": "Polymerase chain reaction (PCR)",
      "wrongOptions": [
        "Single-Strand Binding Proteins (SSBs)",
        "DNA Gyrase (Topoisomerase)",
        "5' to 3' direction"
      ]
    },
    {
      "number": 17,
      "question": "Which heat-stable DNA polymerase is used in PCR?",
      "answer": "Taq polymerase",
      "wrongOptions": [
        "Lagging Strand",
        "Okazaki Fragments",
        "Leading Strand"
      ]
    },
    {
      "number": 18,
      "question": "What is the technique separating DNA fragments by size?",
      "answer": "Gel electrophoresis",
      "wrongOptions": [
        "Lagging Strand",
        "Taq polymerase",
        "Okazaki Fragments"
      ]
    }
  ]
}