D1.1 DNA Replication
18 review questions with answers for D1.1 DNA Replication, marked SL and HL. Read them here, or play the set as a class game on the projector. Free to host, and students join with a code.
The direction in which DNA Polymerase always adds new nucleotides?
- Gel electrophoresis
- DNA Polymerase I
- 5' to 3' directionRight answer
- Polymerase chain reaction (PCR)
All 18 questions and answers
The direction in which DNA Polymerase always adds new nucleotides?
Answer: 5' to 3' direction
The enzyme that relieves torsional strain (overwinding) ahead of the replication fork?
Answer: DNA Gyrase (Topoisomerase)
Proteins that bind to the DNA strands after they are separated to prevent them from re-annealing?
Answer: Single-Strand Binding Proteins (SSBs)
The enzyme that creates a short RNA primer to provide an attachment point for DNA Polymerase?
Answer: DNA Primase
The strand that is synthesized continuously moving toward the replication fork?
Answer: Leading Strand
The strand that is synthesized discontinuously in fragments moving away from the fork?
Answer: Lagging Strand
Short lengths of DNA produced on the lagging strand?
Answer: Okazaki Fragments
The enzyme that joins Okazaki fragments together by forming phosphodiester bonds?
Answer: DNA Ligase
The enzyme that removes the RNA primers and replaces them with DNA?
Answer: DNA Polymerase I
The term describing DNA replication because each new molecule contains one original and one new strand?
Answer: Semi-conservative
The enzyme that unwinds the double helix and breaks hydrogen bonds between bases?
Answer: Helicase
Main enzyme in prokaryotes that adds nucleotides to the 3' end of the primer to build the new strand?
Answer: DNA Polymerase III
The experiment that used ¹⁵N and ¹⁴N to show that DNA replication is semi-conservative?
Answer: Meselson-Stahl Experiment
The technique used in the Meselson-Stahl lab to separate DNA based on density?
Answer: Centrifugation
The way in which DNA bases pair: A with T and G with C?
Answer: Complementary base pairing
What is the technique used to amplify DNA?
Answer: Polymerase chain reaction (PCR)
Which heat-stable DNA polymerase is used in PCR?
Answer: Taq polymerase
What is the technique separating DNA fragments by size?
Answer: Gel electrophoresis
Play it as a class game
Play this set opens it in splycr, where you can host it for your class straight away. Hosting needs no account: put the room code on the projector, and students join from a phone with the code and their first name. Each question plays as multiple choice, and the three wrong options are other answers from the same set.